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The Water Cooler
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CDC admits death toll is inflated! Of 161,392 deaths ONLY 6% / 9,683 ARE DIRECTLY CAUSED BY COVID.
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<blockquote data-quote="_CY_" data-source="post: 3544026" data-attributes="member: 7629"><p>sauce for above .. </p><p></p><p>2020 Dec 13</p><p><span style="font-size: 22px"><strong>SARS-CoV-2 RNA reverse-transcribed and integrated into the human genome</strong></span></p><p><span style="font-size: 22px"><strong><a href="https://pubmed.ncbi.nlm.nih.gov/33330870/" target="_blank">https://pubmed.ncbi.nlm.nih.gov/33330870/</a></strong></span></p><p><span style="font-size: 22px"></span></p><p><span style="font-size: 22px"><span style="font-size: 18px"><strong>Abstract</strong></span></span></p><p><span style="font-size: 22px">Prolonged SARS-CoV-2 RNA shedding and recurrence of PCR-positive tests have been widely reported in patients after recovery, yet these patients most commonly are non-infectious. Here we investigated the possibility that SARS-CoV-2 RNAs can be reverse-transcribed and integrated into the human genome and that transcription of the integrated sequences might account for PCR-positive tests. In support of this hypothesis, we found chimeric transcripts consisting of viral fused to cellular sequences in published data sets of SARS-CoV-2 infected cultured cells and primary cells of patients, consistent with the transcription of viral sequences integrated into the genome. To experimentally corroborate the possibility of viral retro-integration, we describe evidence that SARS-CoV-2 RNAs can be reverse transcribed in human cells by reverse transcriptase (RT) from LINE-1 elements or by HIV-1 RT, and that these DNA sequences can be integrated into the cell genome and subsequently be transcribed. Human endogenous LINE-1 expression was induced upon SARS-CoV-2 infection or by cytokine exposure in cultured cells, suggesting a molecular mechanism for SARS-CoV-2 retro-integration in patients. This novel feature of SARS-CoV-2 infection may explain why patients can continue to produce viral RNA after recovery and suggests a new aspect of RNA virus replication.</span></p><p><span style="font-size: 22px"></span></p><p><span style="font-size: 22px"></span></p></blockquote><p></p>
[QUOTE="_CY_, post: 3544026, member: 7629"] sauce for above .. 2020 Dec 13 [SIZE=6][B]SARS-CoV-2 RNA reverse-transcribed and integrated into the human genome[/B] [B][URL]https://pubmed.ncbi.nlm.nih.gov/33330870/[/URL][/B] [SIZE=5][B]Abstract[/B][/SIZE][/SIZE] [SIZE=6]Prolonged SARS-CoV-2 RNA shedding and recurrence of PCR-positive tests have been widely reported in patients after recovery, yet these patients most commonly are non-infectious. Here we investigated the possibility that SARS-CoV-2 RNAs can be reverse-transcribed and integrated into the human genome and that transcription of the integrated sequences might account for PCR-positive tests. In support of this hypothesis, we found chimeric transcripts consisting of viral fused to cellular sequences in published data sets of SARS-CoV-2 infected cultured cells and primary cells of patients, consistent with the transcription of viral sequences integrated into the genome. To experimentally corroborate the possibility of viral retro-integration, we describe evidence that SARS-CoV-2 RNAs can be reverse transcribed in human cells by reverse transcriptase (RT) from LINE-1 elements or by HIV-1 RT, and that these DNA sequences can be integrated into the cell genome and subsequently be transcribed. Human endogenous LINE-1 expression was induced upon SARS-CoV-2 infection or by cytokine exposure in cultured cells, suggesting a molecular mechanism for SARS-CoV-2 retro-integration in patients. This novel feature of SARS-CoV-2 infection may explain why patients can continue to produce viral RNA after recovery and suggests a new aspect of RNA virus replication. [/SIZE] [/QUOTE]
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CDC admits death toll is inflated! Of 161,392 deaths ONLY 6% / 9,683 ARE DIRECTLY CAUSED BY COVID.
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